The determination of the moisture content of all malts by loss in mass on drying under specified conditions. The ISO Method for moisture content of grain (ISO 712-1985) which is used for barley (EBC Method 3.2) must not be used for malt as the conditions of drying are such that chemical condensations occur in which water is formed as a product of reaction.
It is necessary to use EBC Method 1.1 and 4.1
Descriptors: general anaerobic count on samples of yeast or fermenting beer.
It is necessary to use EBC Microbiology 4.2.4.1 to 4.2.4.3
Quantitative detection of Enterobacteriaceae in yeast. Descriptors: selective media for the growth of Enterobacteriaceae.
Quantitative detection of bacteria belonging to the genera Lactobacillus and Pediococcus. No single method is known that is capable of detecting all strains of Lactic Acid Bacteria. Descriptors: plating method.
It is necessary to use EBC Microbiology 2.3.3.2, 2.3.4.3 or 2.3.4.4, 2.3.5.4 and 2.3.5.1, 2.3.7 and 2.3.6.1 or 2.3.6.2.
The detection and enumeration of acetic acid bacteria in fermenting beer. Descriptors: Acetobacter and Gluconobacter; vinegar odour.
It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2
Detection of Saccharomyces wild yeasts in brewers' yeast and fermenting beer. Descriptors: Cu-differentiation, aerobic incubation.
It is necesssary to use EBC Microbiology 2.3.3.1 or 2.3.3.2, 2.3.4.2 and 2.3.5.1
Detection of Saccharomyces cerevisiae ‘ale’ yeasts in a population of Saccharomyces pastorianus (syn. S. carslbergensis) ‘lager’ yeast. Descriptors: Heat Differentiation, Saccharomyces wild yeasts, Saccharomyces bottom fermenting brewers' yeast.
It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2 and 2.3.4.2
Detection and enumeration of non-Saccharomyces yeasts in fermenting beer or other yeast containing samples. Descriptors: plating technique, nitrogen requirements, lysine plates.
Quantitative detection of Dekkera wild yeasts in samples of brewers' yeast, fermenting beer and filtered beer. Descriptors: Dekkera (formerly Brettanomyces); Dekkera medium, produce acetic acid.
It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2, 2.3.4.2 and 2.3.5.4 and 1.4.3
EBC Method 4.2.7.1 Dekkera (formerly Brettanomyces) Detection with FastOrangeÒ Brett Medium (IM) from 2021 allows for the detection of super-attenuating Dekkera yeasts – formerly Brettanomyces – in brewery samples or within other yeasts on FastOrangeÒ BRETT agar medium.
Determination of the biological stability of bottled beer at room temperature. Descriptors: Shelf Life of Beer in Bottles, growth of microorganisms
Determination of the biological stability of beer at room temperature. This method is applicable to a package that is not penetrable to light, such as can, keg or shielded bottle. It is also applicable to dark beer. Descriptors; shelf life of beer in cans and kegs, growth of microorganisms, off-flavour.
Determination of the biological stability of beer at room temperature. The method is applicable to kegged beer. Descriptors: shelf life of kegged beer transferred to bottles, growth of microorganisms.
It is necessary to use EBC Microbiology 2.2.1.5 and 4.3.1.1
Qualitative detection of microorganisms in bottled beer. Descriptors: enrichment, growth of microorganisms.
It is necessary to use EBC Microbiology 4.3.1.1
The determination of the total nitrogen content of malt by a Kjeldahl procedure.
It is necessary to use EBC Method 3.3.1
Quantitative evaluation of aerobic and facultative microorganisms in filtered beer, recovered beer, beer in keg, etc. Descriptors: general aerobic count on beer, growth of microorganisms.
It is necesary to use EBC Microbiology 2.3.2.1 and 2.3.3.2 and 2.3.4.2
Quantitative evaluation of anaerobic microorganisms in filtered beer, recovered beer, beer in keg, etc. Descriptors: general anaerobic count on beer, growth of microorganisms.
It is necessary to use EBC Microbiology 2.3.2.1 and 2.3.2.2 and 2.3.4.3 or 2.3.4.4
The determination of the total nitrogen content of malt by a combustion method based on the Dumas principle.
It is necessary to use EBC Method 3.3.2
Quantitative detection of bacteria belonging to the genera Lactobacillus and Pediococcus. Descriptors. lactic acid bacteria, selective culture medium, microscopy, gram differentiation, catalase test, membrane filtration, incubation.
It is necessary to use EBC Microbiology 2.3.2.1, 2.3.2.2, 2.3.4.3, 2.3.5.4, 2.3.6.1 or 2.3.6.2 and 2.3.7
Quantitative detection of strictly anaerobic Pectinatus and Megasphaera in bottled beer. Pectinatus and Megasphaera may arise as secondary contaminants when tunnel pasteurisation of the packaged product is not applied. Descriptors: anaerobic incubation, microscopy, selective medium.
It is necessary to use EBC Microbiology 2.3.7 and 2.3.6.1 and 2.3.6.2
Qualitative detection of strictly anaerobic Pectinatus and Megasphaera in bottled beer. Pectinatus and Megasphaera may arise as secondary contaminants when tunnel pasteurisation of the packaged product is not applied. Descriptors: selective enrichment, anaerobic incubation.
It is necessary to use EBC Microbiology 2.3.5.1, 2.3.5.2, 2.3.5.3 and 2.3.5.4 and 2.3.6.1 and 2.3.6.2
Detection of culturable water bacteria. Descriptors: growth medium. For full details see: EN ISO 6222:2000 Enumeration of culturable micro-organisms — colony count by inoculation in a nutrient agar culture medium.
It is necessary to use EBC Microbiology 2.3.3.1 and 2.3.4.2
Detection and enumeration of Escherichia coli and coliform bacteria in potable water for human consumption. Because of the low selectivity, the method is unsuitable for water that has not been disinfected. Descriptors: membrane filtration, selective medium. For full details see: EN ISO 9308-1: 2000 Water quality — detection and enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method.
Detection and enumeration of intestinal enterococci in drinking water. Descriptors: membrane filtration, incubation. For full details see: ISO 7899-2:2000 Water quality — detection and enumeration of intestinal enterococci Part 2: Membrane filtration method.
Detection and enumeration of Escherichia coli and coliform bacteria in water for human consumption. Descriptors: most probable number (MPN), chromogenic / fluorogenic method. EBC method 4.4.2 “Escherichia coli and Coliform Bacteria”, is based on EN ISO 9308-1: 2000 ‘Water quality — detection and enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method’. This method has been reported to have low selectivity and is not well suited for routine laboratory analysis of water. The following methodology is now accepted in most countries as a national alternative to the EN ISO method.
It is necessary to use EBC Microbiology 2.2.3.4
The determination of average corn weight as a measure of barley quality.
It is necessary to use EBC Method 3.4
Detection and enumeration of Clostridium perfringens (including spores) in water. Descriptors: membrane filtration, selective medium. As stated in : Council directive 98/83/EC On the quality of water intended for human consumption : Annex III Specifications for the analysis of parameters.
The detection and confirmation of Fusarium in barley and malt. Descriptors: kernels incubation, selective medium.
The determination of the potential of malt for producing wort solubles by a standard mashing programme. This procedure is also used for the determination of saccharification rate, odour, speed of filtration, pH of wort, colour, viscosity of wort, soluble nitrogen content and free amino nitrogen content.
It is necessary to use EBC Methods 1.1 , 8.2.1 or 8.2.2, 4.7.1 or 4.7.2 and 8.3
The detection and confirmation of storage fungi (Penicillium and Aspergillus) in barley and malt. Descriptors: incubation of kernels, storage fungi, surface disinfection.
