Analytica EBC methods list - page 6
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ANALYTICA EBC

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Total aerobic count of yeast and bacteria in malt and barley
  • 26/10/2018
  • 3220
  • |
  • Detection of Contaminants
  • |
  • 2011

4.5.6 - Total aerobic count of yeast and bacteria in malt and barley

Any sample of barley or malted barley. This method can also be applied to other dry grains such as wheat, and is most useful for trending of results over a period of time rather than for absolute analysis. Descriptors: total aerobic count of yeast and bacteria in malt and barley, serial dilution.

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Analytica EBC - Detection of Contaminants in Additives
  • 26/10/2018
  • 2890
  • |
  • Detection of Contaminants
  • |
  • 2011

4.6.1 - Detection of Contaminants in Additives

The detection of brewery contaminants in additives. Descriptors: selective media, incubation conditions, count the number of colonies, colony forming units (CFU’s).

It is necessary to use EBC Microbiology 2.3.3.1, 2.3.3.2, 2.3.4.2, 2.3.4.3 and 2.3.4.4

Analytica EBC - Hot Water Extract of Malt: Constant Temperature Mash
  • 22/10/2018
  • 6135
  • |
  • Malt
  • |
  • 1997

4.6.1 - Hot Water Extract of Malt: Constant Temperature Mash

The determination of Hot Water Extract of ale, lager and distilling malts using a constant temperature infusion mashing procedure of a coarsely ground sample (0,7 mm). The wort produced by this mashing procedure is used for ancillary analyses, such as the determination of saccharification rate, colour, viscosity of wort, soluble nitrogen content, free amino nitrogen content, fermentability, and pH. Fine/coarse difference may be derived by simultaneously determining the Hot Water Extract of a more finely ground grist (0,2 mm) from a second test portion of the malt sample.

It is necessary to use EBC Methods 1.1 and 4.1

The European reference Analytical Methods for breweries by EBC
Analytica EBC - General Aerobic Count in Dilute Sugars
  • 26/10/2018
  • 3072
  • |
  • Detection of Contaminants
  • |
  • 2011

4.6.2 - General Aerobic Count in Dilute Sugars

The method is used to obtain a count of potential beer spoilage organisms in dilute sugars when a low level of contamination is expected. Microorganisms are concentrated by membrane filtration. Descriptors: general aerobic count in dilute sugars, nutrient medium.

It is necessary to use EBC Microbiology 2.3.2.1

Analytica EBC - General Aerobic Count in Process Gases
  • 26/10/2018
  • 3136
  • |
  • Detection of Contaminants
  • |
  • 2011

4.6.3 - General Aerobic Count in Process Gases

To assess the microbiological quality of any process gas. This method is used to obtain a total count when small numbers of microorganisms are expected. Descriptors: general aerobic count in process gases, nutrient medium, CFU, membrane filtration.

It is necessary to use EBC Microbiology 2.2.4.1 and 2.3.2.1

Analytica EBC - General Aerobic Count in Acid and Caustic Recovery Systems
  • 26/10/2018
  • 3229
  • |
  • Detection of Contaminants
  • |
  • 2011

4.7.1 - General Aerobic Count in Acid and Caustic Recovery Systems

Quantitative evaluation of aerobic and facultative microorganisms in caustic or acid solutions of CIP recovery systems. Both acid and caustic recovery systems can become contaminated and it is recommended that tanks used to recover acid or caustic should be regularly assessed for this reason. Descriptors: general aerobic count in acid and caustic recovery systems.

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Analytica EBC - Colour of Malt. Spectrophotometric Method (RM)
  • 22/10/2018
  • 6675
  • |
  • Malt
  • |
  • GM
  • 2000

4.7.1 - Colour of Malt. Spectrophotometric Method (RM)

The determination of the colour of worts produced from a laboratory extract of malt by a spectrophotometric method. This method has been adopted as the reference method in preference to the more subjective visual method.

It is necessary to use EBC Methods 4.5.1 or 4.6.1 and 4.6.2

Analytica EBC - Microbiological Evaluation of the Efficacy of Sterilants and Biocides
  • 26/10/2018
  • 2956
  • |
  • Detection of Contaminants
  • |
  • 2011

4.7.2 - Microbiological Evaluation of the Efficacy of Sterilants and Biocides

The efficacy of chemical disinfectants should preferably be tested according to the approved methods in the guidelines of the European Committee for Standardisation (CEN). Descriptors: efficacy of disinfectants, bovine albumin, standardised hard water, European standards for testing chemical disinfectants, microbiological evaluation of the efficacy of sterilants and biocides.

Analytica EBC - Colour of Malt: Visual Method
  • 22/10/2018
  • 5494
  • |
  • Malt
  • |
  • 1997

4.7.2 - Colour of Malt: Visual Method

The determination of colour in malt wort using a colour comparator. Descriptors: transmission of the ebc scale glasses

it is necessary to use EBC Method 4.1

Analytica EBC - Detection of Saccharomyces Cerevisiae var. diastaticus
  • 15/04/2024
  • 578
  • |
  • Detection of Contaminants
  • |
  • GM
  • 2024

4.8 - Detection of Saccharomyces Cerevisiae var. diastaticus

Detection of Saccharomyces cerevisiae var. diastaticus in samples of brewers' yeast, fermenting, filtered and packaged beer and the production environment including water, swabs and air.

Descriptors: Saccharomyces cerevisiae var. diastaticus, super attenuation, over carbonation, spoilage, wild yeast, PCR.

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Viscosity of Laboratory Wort from Malt
  • 22/10/2018
  • 4329
  • |
  • Malt
  • |
  • 2004

4.8 - Viscosity of Laboratory Wort from Malt

The determination of viscosity of worts produced during the course of malt analysis, using an appropriate viscometer.

It is necessary to use EBC Method 8.4

Analytica EBC - Soluble Nitrogen of Malt: Kjeldahl Method
  • 22/10/2018
  • 4858
  • |
  • Malt
  • |
  • 1997

4.9.1 - Soluble Nitrogen of Malt: Kjeldahl Method

The determination of the total nitrogen content of won, prepared during the course of malt analysis, by a Kjeldahl procedure.

It is necessary to use EBC Methods 3.3.1 and 4.5.1

Analytica EBC - Soluble Nitrogen of Malt: Dumas Combustion Method
  • 22/10/2018
  • 3806
  • |
  • Malt
  • |
  • 1999

4.9.3 - Soluble Nitrogen of Malt: Dumas Combustion Method

The determination of the total nitrogen content in wort, prepared during the course of malt analysis, by the Dumas combustion method. Descriptors: calculate the soluble nitrogen content as a percentage of dry malt.

it is necessary to use EBC Methods 4.2, 4.5.1 or 4.6

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Free Amino Nitrogen of Malt by Spectrophotometry
  • 22/10/2018
  • 4780
  • |
  • Malt
  • |
  • 1997

4.10 - Free Amino Nitrogen of Malt by Spectrophotometry

The determination of the free amino nitrogen content of malt using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-­amino nitrogen since γ-amino butyric acid, which is present in malt worts, also gives a colour reaction with ninhydrin.

It is necessary to use EBC Methods 4.5.1 or 4.6.1 and 8.10

Analytica EBC - Fermentability, Final Attenuation of Laboratory Wort from Malt: Reference Method
  • 22/10/2018
  • 4216
  • |
  • Malt
  • |
  • 1999

4.11.1 - Fermentability, Final Attenuation of Laboratory Wort from Malt: Reference Method

The determination of the fermentability of wort using a yeast procedure. Descriptors: fermentability from the equation, original gravity of the wort and the present gravity of the fermented filtrate, calculate the percentage apparent attenuation, percentage real attenuation, fermentable extract

It is necessary to use EBC Methods 4.1, 4.5.1 or 4.6.1

Analytica EBC - Fermentability, Final Attenuation of Laboratory Wort from Malt: Rapid
  • 22/10/2018
  • 3577
  • |
  • Malt
  • |
  • 1999

4.11.2 - Fermentability, Final Attenuation of Laboratory Wort from Malt: Rapid

The determination of the fermentabílity of wort using a rapid yeast procedure.  This method is given for guideline purposes only. It must be considered that the rapid method may lead to lower results compared to the reference method. Descriptors: original gravity of the wort and the present gravity of the fermented filtrate, percentage apparent attenuation, percentage real attenuation.

It is necessary to use EBC Methods 4.1, 4.5.1 or 4.6.1

The European reference Analytical Methods for breweries by EBC
The European reference Analytical Methods for breweries by EBC
Analytica EBC - α-Amylase Content of Malt (IM)
  • 22/10/2018
  • 4610
  • |
  • Malt
  • |
  • IM
  • 2006

4.13 - α-Amylase Content of Malt (IM)

The determination of the α-amylase activity of malt as the dextrinization time of a standardized starch solution in the presence of excess β-amylase.

It is necessary to use EBC Method 1.1

Analytica EBC - Modification and Homogeneity of Malt: Calcofluor Method
  • 28/10/2020
  • 5144
  • |
  • Malt
  • |
  • 2020

4.14 - Modification and Homogeneity of Malt: Calcofluor Method

Estimation of the average modification as a percentage and the homogeneity of barley malt. Descriptors: MicroFluo calculation file, modification, variance, square of the standard deviation, homogeneity.

The updated method (2020) encompasses new coloration conditions.

It is necessary to use EBC Method 4.1

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