The determination of the amount of fermentable sugars in the wort using a rapid yeast procedure. This rapid method is given for guideline purposes only. It must be taken into consideration that the rapid method may lead to lower results compared to the reference method (EBC Method 8.6.1).
The determination of fructose, glucose and sugars with 2 and 3 degrees of polymerization (DP2 and DP3) by high performance liquid chromatography (HPLC). The DP2 peak is primarily maltose, while the DP3 peak is primarily maltotriose. The method cannot distinguish between different disaccharides or between different trisaccharides. Flow rate and column temperature are considered to be sensitive parameters. Deviating from the procedure may alter the results.
The determination of the bitter substances, mainly iso- α-acids, in wort.
It is necessary to use EBC Method 9.8
The determination of total nitrogen content of wort by a Kjeldahl procedure.
It is necessary to use EBC Method 3.3.1
The determination of the total nitrogen content in wort, by the Dumas combustion method.
It is necessary to use EBC Method 9.9.2
The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.
The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.
The determination of zinc in wort by atomic absorption spectrophotometry. Descriptors: calculation of the level of zinc obtained by atomic absorption spectrophotometry
The determination of total polyphenols in wort by spectrophotometry.
It is necessary to use EBC Method 9.11
Determination of the high molecular weight mixed linkage (1,3) (1,4) ß-D glucan (ß-glucan) fraction present in wort.
It is necessary to use EBC Method 3.10.1
Determination of the high molecular weight (1,3)(1,4) β-D glucan (β-glucan) fraction present in wort.
It is necessary to use EBC Method 3.10.2
The determination of the total carbohydrate content of wort by spectrophotometry.
Conductometric method for the determination of chloride in wort by precipitation as silver chloride.
It is necessary to use EBC Method 9.21
Gravimetric determination of sulphate with barium chloride.
It is necessary to use EBC Method 9.22
The determination of pH at 20 °C of wort; using a pH meter and a suitable electrode system.
It is necessary to use EBC Method 1.5
The determination of calcium in wort by atomic absorption spectrophotometry.
It is necessary to use EBC Method 1.3
The determination of dissolved oxygen in wort using optical oxygen sensors. Descriptors: solubility of oxygen in water in equilibrium with air at 1,01325 bar and vapour pressure of water in equilibrium with air, solubility of oxygen.
it is necessary to use EBC Method 9.37.1
The determination of dissolved oxygen in wort using electrochemical oxygen sensors.
It is necessary to use EBC Method 9.37
Procedure to obtain a homogenous and representative sample of beer before filling for testing.
The determination of the alcohol content of beer using a distillation procedure and measurement of specific gravity of the distillate. Alternative procedures may produce accurate results but the distillation procedure is regarded as the reference method for alcohol.
It is necessary to use EBC Method 9.43.1 and 9.46
The rapid determination of the alcohol content of beer using an instrument relying on catalytic combustion. The description of the method is intended only as a guide since the instrumental procedure differs from model to model.
It is necessary to use EBC Method 9.2.1
The determination of the alcohol content of beer by refractometry.
It is necessary to use EBC Method 9.2.1 and 9.43.1
The determination of the ethanol content of beer by gas liquid chromatography using direct liquid injection.
It is necessary to use EBC Method 9.2.1 and 9.43.1
A procedure to correct the alcohol content of a microbiologically spoiled beer for volatile acidity. Descriptors: volatile acid alcohol equivalent.
it is necessary to use EBC Method 9.2.1 and 9.2.4
The rapid determination of the alcohol content of beer using an automated instrument relying on near infrared spectroscopy (NIR). The description of the method is intended only as a guide since there are different types of NIR equipment available and the instrumental procedure differs from model to model.
It is necessary to use EBC Method 9.2.1 and 9.43.1 or 9.43.2
The determination of ethanol in beer by enzymatic analysis.
The determination of the ethanol content of alcohol free and low alcohol beers by gas liquid chromatography using headspace injection and flame ionization detection.
The calculation of original, real and apparent extract and original gravity of beer from specific gravity determinations obtained on the beer and on the alcoholic distillate and beer residue after distillation.
It is necessary to use EBC Methods 9.2.1 and 9.43.2 or 9.43.1
The determination of real degree of fermentation by calculation from measurements of alcohol content and real extract.
It is necessary to use EBC Methods 9.2.1 and 9.4
The determination of the colour of beer by spectrophotometry.
It is necessary to use EBC Method 8.5
