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ANALYTICA EBC

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Fermentability, Attenuation Limit of Wort Rapid Fermentation
  • 23/10/2018
  • 1557
  • |
  • Wort
  • |
  • 2002

8.6.2 - Fermentability, Attenuation Limit of Wort Rapid Fermentation

The determination of the amount of fermentable sugars in the wort using a rapid yeast procedure. This rapid method is given for guideline purposes only. It must be taken into consideration that the rapid method may lead to lower results compared to the reference method (EBC Method 8.6.1).

Analytica EBC - Fermentable Carbohydrates in Wort by HPLC (IM)
  • 23/10/2018
  • 1451
  • |
  • Wort
  • |
  • IM
  • 2004

8.7 - Fermentable Carbohydrates in Wort by HPLC (IM)

The determination of fructose, glucose and sugars with 2 and 3 degrees of polymerization (DP2 and DP3) by high performance liquid chromatography (HPLC). The DP2 peak is primarily maltose, while the DP3 peak is primarily maltotriose. The method cannot distinguish between different disaccharides or between different trisaccharides. Flow rate and column temperature are considered to be sensitive parameters. Deviating from the procedure may alter the results.

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Bitterness of Wort
  • 23/10/2018
  • 1604
  • |
  • Wort
  • |
  • IM
  • 2003

8.8 - Bitterness of Wort

The determination of the bitter substances, mainly iso- α-acids, in wort.

It is necessary to use EBC Method 9.8

Analytica EBC - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)
  • 23/10/2018
  • 1992
  • |
  • Wort
  • |
  • IM
  • 2015

8.10.1 - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Free Amino Nitrogen in Wort by Segmented Flow Analysis
  • 23/10/2018
  • 1271
  • |
  • Wort
  • |
  • VM
  • 2011

8.10.2 - Free Amino Nitrogen in Wort by Segmented Flow Analysis

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

Analytica EBC - Free amino nitrogen in wort by automated discrete analysis (IM)
  • 01/07/2021
  • 522
  • |
  • Wort
  • |
  • IM
  • 2021

8.10.3 - Free amino nitrogen in wort by automated discrete analysis (IM)

EBC Method 8.10.3 Free Amino Nitrogen in Wort by Automated Discrete Analysis (IM) – 2021 allows for the determination of the free amino nitrogen content in wort using photometric measurement of OPA (oPhthaldialdehyde) and NAC (N-acetyl cysteine). The method is performed manually or automatically at a 340 nm wavelength at 37 °C temperature.

The European reference Analytical Methods for breweries by EBC
The European reference Analytical Methods for breweries by EBC
Analytica EBC - pH of Wort
  • 23/10/2018
  • 1533
  • |
  • Wort
  • |
  • 1999

8.17 - pH of Wort

The determination of pH at 20 °C of wort; using a pH meter and a suitable electrode system.

It is necessary to use EBC Method 1.5

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Dissolved Oxygen in Wort using Optical Sensors
  • 23/10/2018
  • 1145
  • |
  • Wort
  • |
  • 2015

8.19.1 - Dissolved Oxygen in Wort using Optical Sensors

The determination of dissolved oxygen in wort using optical oxygen sensors. Descriptors: solubility of oxygen in water in equilibrium with air at 1,01325 bar and vapour pressure of water in equilibrium with air, solubility of oxygen.

it is necessary to use EBC Method 9.37.1

Analytica EBC - Alcohol in Beer by Distillation
  • 23/10/2018
  • 3297
  • |
  • Beer
  • |
  • 2008

9.2.1 - Alcohol in Beer by Distillation

The determination of the alcohol content of beer using a distillation procedure and measurement of specific gravity of the distillate. Alternative procedures may produce accurate results but the distillation procedure is regarded as the reference method for alcohol.

It is necessary to use EBC Method 9.43.1 and 9.46

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Alcohol in Beer by Catalytic Combustion
  • 23/10/2018
  • 1245
  • |
  • Beer
  • |
  • AM
  • 2007

9.2.2 - Alcohol in Beer by Catalytic Combustion

The rapid determination of the alcohol content of beer using an instrument relying on catalytic combustion. The description of the method is intended only as a guide since the instrumental procedure differs from model to model.

It is necessary to use EBC Method 9.2.1

Analytica EBC - Ethanol in Beer by Gas Chromatography
  • 23/10/2018
  • 1563
  • |
  • Beer
  • |
  • 2007

9.2.4 - Ethanol in Beer by Gas Chromatography

The determination of the ethanol content of beer by gas liquid chromatography using direct liquid injection.

It is necessary to use EBC Method 9.2.1 and 9.43.1

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Alcohol in Beer by Near Infrared Spectroscopy
  • 23/10/2018
  • 1933
  • |
  • Beer
  • |
  • 2008

9.2.6 - Alcohol in Beer by Near Infrared Spectroscopy

The rapid determination of the alcohol content of beer using an automated instrument relying on near infrared spectroscopy (NIR). The description of the method is intended only as a guide since there are different types of NIR equipment available and the instrumental procedure differs from model to model.

It is necessary to use EBC Method 9.2.1 and 9.43.1 or 9.43.2

Analytica EBC

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