Analytica EBC methods list - page 9
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ANALYTICA EBC

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Sampling of Wort
  • 23/10/2018
  • 3052
  • |
  • Wort
  • |
  • 1999

8.1 - Sampling of Wort

This method describes procedures employed to obtain homogeneous and representative samples of wort for analysis.

Analytica EBC - Specific Gravity of Wort using a Pyknometer
  • 23/10/2018
  • 3739
  • |
  • Wort
  • |
  • 2004

8.2.1 - Specific Gravity of Wort using a Pyknometer

The determination of the specific gravity and the defined specific gravity/density derived values of wort in air at 20 °C by weighing a known volume in a pyknometer or gravity bottle. Descriptors: pyknometer, reischauer type, gay-lussac type, gravity bottle.

Analytica EBC - Specific Gravity of Wort using a Density Meter
  • 23/10/2018
  • 3355
  • |
  • Wort
  • |
  • 2004

8.2.2 - Specific Gravity of Wort using a Density Meter

The measurement of the specific gravity and the defined specific gravity/density derived values of a wort at 20 °C using digital density meters of the oscillation type.

it is necessary to use EBC Methods 1.6 and 8.3

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Fermentability, Attenuation Limit of Wort Rapid Fermentation
  • 23/10/2018
  • 3444
  • |
  • Wort
  • |
  • 2002

8.6.2 - Fermentability, Attenuation Limit of Wort Rapid Fermentation

The determination of the amount of fermentable sugars in the wort using a rapid yeast procedure. This rapid method is given for guideline purposes only. It must be taken into consideration that the rapid method may lead to lower results compared to the reference method (EBC Method 8.6.1).

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Analytica EBC - Fermentable Carbohydrates in Wort by HPLC (IM)
  • 23/10/2018
  • 3275
  • |
  • Wort
  • |
  • IM
  • 2004

8.7 - Fermentable Carbohydrates in Wort by HPLC (IM)

The determination of fructose, glucose and sugars with 2 and 3 degrees of polymerization (DP2 and DP3) by high performance liquid chromatography (HPLC). The DP2 peak is primarily maltose, while the DP3 peak is primarily maltotriose. The method cannot distinguish between different disaccharides or between different trisaccharides. Flow rate and column temperature are considered to be sensitive parameters. Deviating from the procedure may alter the results.

Analytica EBC - Bitterness of Wort
  • 23/10/2018
  • 3775
  • |
  • Wort
  • |
  • IM
  • 2003

8.8 - Bitterness of Wort

The determination of the bitter substances, mainly iso- α-acids, in wort.

It is necessary to use EBC Method 9.8

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Analytica EBC - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)
  • 23/10/2018
  • 4238
  • |
  • Wort
  • |
  • IM
  • 2015

8.10.1 - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

Analytica EBC - Free Amino Nitrogen in Wort by Segmented Flow Analysis
  • 23/10/2018
  • 2690
  • |
  • Wort
  • |
  • VM
  • 2011

8.10.2 - Free Amino Nitrogen in Wort by Segmented Flow Analysis

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

Analytica EBC - Free amino nitrogen in wort by automated discrete analysis (IM)
  • 01/07/2021
  • 1931
  • |
  • Wort
  • |
  • IM
  • 2021

8.10.3 - Free amino nitrogen in wort by automated discrete analysis (IM)

EBC Method 8.10.3 Free Amino Nitrogen in Wort by Automated Discrete Analysis (IM) – 2021 allows for the determination of the free amino nitrogen content in wort using photometric measurement of OPA (oPhthaldialdehyde) and NAC (N-acetyl cysteine). The method is performed manually or automatically at a 340 nm wavelength at 37 °C temperature.

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Analytica EBC - pH of Wort
  • 23/10/2018
  • 3477
  • |
  • Wort
  • |
  • 1999

8.17 - pH of Wort

The determination of pH at 20 °C of wort; using a pH meter and a suitable electrode system.

It is necessary to use EBC Method 1.5

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Analytica EBC - Dissolved Oxygen in Wort using Optical Sensors
  • 23/10/2018
  • 2327
  • |
  • Wort
  • |
  • 2015

8.19.1 - Dissolved Oxygen in Wort using Optical Sensors

The determination of dissolved oxygen in wort using optical oxygen sensors. Descriptors: solubility of oxygen in water in equilibrium with air at 1,01325 bar and vapour pressure of water in equilibrium with air, solubility of oxygen.

it is necessary to use EBC Method 9.37.1

Analytica EBC - Iso-alpha-acids and alpha-acids in wort by HPLC
  • 30/09/2022
  • 580
  • |
  • Wort
  • |
  • GM
  • 2022

8.20 - Iso-alpha-acids and alpha-acids in wort by HPLC

This method specifies a high performance liquid chromatographic technique for the simultaneous determination of iso-α-acids (iso) and α-acids (alpha) in wort. It is recommended for wort samples containing α-acids and iso-α-acids. Due to the coelution of iso-α-acids (or α-acids) and reduced iso-α-acids, this method is not recommended for the analysis of wort samples containing reduced iso-α-acids.

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Analytica EBC - Alcohol in Beer by Distillation
  • 23/10/2018
  • 6850
  • |
  • Beer
  • |
  • 2008

9.2.1 - Alcohol in Beer by Distillation

The determination of the alcohol content of beer using a distillation procedure and measurement of specific gravity of the distillate. Alternative procedures may produce accurate results but the distillation procedure is regarded as the reference method for alcohol.

It is necessary to use EBC Method 9.43.1 and 9.46

Analytica EBC

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