ANALYTICA EBC

• 26/10/2018
• 3752
• |
• Detection of Contaminants
• |
• 2011

4.1.1 - Wort Forcing Test

Qualitative detection of microorganisms present and able to grow in wort. Descriptors: wort forcing test, detection of contaminants in wort, haze and CO2 development.

• 26/10/2018
• 3250
• |
• Detection of Contaminants
• |
• 2011

4.1.2 - General Aerobic Count

Quantitative detection of aerobic and facultative microorganisms in wort. Descriptors: general aerobic count, aerobic incubation, growth, Plating method, partially selective or non-selective.

It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 3075
• |
• Detection of Contaminants
• |
• 2011

4.1.3.1 - Enterobacteriaceae

Quantitative detection of Enterobacteriaceae in wort. Descriptors: selective media for the growth of Enterobacteriaceae, aerobic incubation.

• 26/10/2018
• 3026
• |
• Detection of Contaminants
• |
• 2011

4.1.3.2 - Lactic Acid Bacteria

Quantitative detection of lactic acid bacteria in wort. Descriptors. contaminants, differentiated into rods or cocci, incubation.

It is necessary to use EBC Method 2.3.3.2 and 2.3.4.3 or 2.3.4.4 and 2.3.5.1 or 2.3.5.2 or 2.3.5.4 and 2.3.7 and 2.3.6.1 or 2.3.6.2.

• 26/10/2018
• 2677
• |
• Detection of Contaminants
• |
• 2011

4.2.1 - Enrichment

Detection of bacteria in yeast or fermenting beer by the addition of growth medium to the sample. Descriptors: enrichment, concentrated medium, growth of contaminant microorganisms.

• 26/10/2018
• 3178
• |
• Detection of Contaminants
• |
• 2011

4.2.2 - General Aerobic Count on Samples of Yeast or Fermenting Beer

Quantitative detection of aerobic and facultative microorganisms in samples where brewer’s yeast is present, for example, yeast slurry from storage vessel, fermentation samples, beer for cask racking, beer in cask, bottled conditioned beer, etc. Descriptors: General Aerobic Count on Samples of Yeast or Fermenting Beer, aerobic incubation, Plating method, cycloheximide, counnt colonies.

it is necessary to use EBC Microbiology 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 2968
• |
• Detection of Contaminants
• |
• 2011

4.2.3 - General Anaerobic Count on Samples of Yeast or Fermenting Beer

Descriptors: general anaerobic count on samples of yeast or fermenting beer.

It is necessary to use EBC Microbiology 4.2.4.1 to 4.2.4.3

• 26/10/2018
• 3160
• |
• Detection of Contaminants
• |
• 2011

4.2.4.1 - Enterobacteriaceae

Quantitative detection of Enterobacteriaceae in yeast. Descriptors: selective media for the growth of Enterobacteriaceae.

• 26/10/2018
• 3055
• |
• Detection of Contaminants
• |
• 2011

4.2.4.2 - Lactobacillus and Pediococcus

Quantitative detection of bacteria belonging to the genera Lactobacillus and Pediococcus. No single method is known that is capable of detecting all strains of Lactic Acid Bacteria. Descriptors: plating method.

It is necessary to use EBC Microbiology 2.3.3.2, 2.3.4.3 or 2.3.4.4, 2.3.5.4 and 2.3.5.1, 2.3.7 and 2.3.6.1 or 2.3.6.2.

• 26/10/2018
• 3090
• |
• Detection of Contaminants
• |
• 2011

4.2.4.3 - Acetic Acid Bacteria

The detection and enumeration of acetic acid bacteria in fermenting beer. Descriptors: Acetobacter and Gluconobacter; vinegar odour.

It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2

• 26/10/2018
• 3761
• |
• Detection of Contaminants
• |
• 2011

4.2.5.1 - Cu-differentiation

Detection of Saccharomyces wild yeasts in brewers' yeast and fermenting beer. Descriptors: Cu-differentiation, aerobic incubation.

It is necesssary to use EBC Microbiology 2.3.3.1 or 2.3.3.2, 2.3.4.2 and 2.3.5.1

• 26/10/2018
• 2876
• |
• Detection of Contaminants
• |
• 2011

4.2.5.2 - Heat Differentiation

Detection of Saccharomyces cerevisiae ‘ale’ yeasts in a population of Saccharomyces pastorianus (syn. S. carslbergensis) ‘lager’ yeast. Descriptors: Heat Differentiation, Saccharomyces wild yeasts, Saccharomyces bottom fermenting brewers' yeast.

It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 3537
• |
• Detection of Contaminants
• |
• 2011

4.2.6 - Non-Saccharomyces Yeasts

Detection and enumeration of non-Saccharomyces yeasts in fermenting beer or other yeast containing samples. Descriptors: plating technique, nitrogen requirements, lysine plates.

• 26/10/2018
• 2843
• |
• Detection of Contaminants
• |
• 2011

4.2.7 - Dekkera (formerly Brettanomyces)

Quantitative detection of Dekkera wild yeasts in samples of brewers' yeast, fermenting beer and filtered beer. Descriptors: Dekkera (formerly Brettanomyces); Dekkera medium, produce acetic acid.

It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2, 2.3.4.2 and 2.3.5.4 and 1.4.3

• 01/07/2021
• 1995
• |
• Detection of Contaminants
• |
• IM
• 2021

4.2.7.1 - Dekkera (formerly Brettanomyces) Detection with FastOrange® BRETT Medium (IM)

EBC Method 4.2.7.1 Dekkera (formerly Brettanomyces) Detection with FastOrangeÒ Brett Medium (IM) from 2021 allows for the detection of super-attenuating Dekkera yeasts – formerly Brettanomyces – in brewery samples or within other yeasts on FastOrangeÒ BRETT agar medium.

• 26/10/2018
• 3331
• |
• Detection of Contaminants
• |
• 2011

4.3.1.1 - Shelf Life of Beer in Bottles

Determination of the biological stability of bottled beer at room temperature. Descriptors: Shelf Life of Beer in Bottles, growth of microorganisms

• 26/10/2018
• 3063
• |
• Detection of Contaminants
• |
• 2011

4.3.1.2 - Shelf Life of Beer in Cans and Kegs

Determination of the biological stability of beer at room temperature. This method is applicable to a package that is not penetrable to light, such as can, keg or shielded bottle. It is also applicable to dark beer. Descriptors; shelf life of beer in cans and kegs, growth of microorganisms, off-flavour.

• 26/10/2018
• 2440
• |
• Detection of Contaminants
• |
• 2011

4.3.1.3 - Shelf Life of Kegged Beer Transferred to Bottles

Determination of the biological stability of beer at room temperature. The method is applicable to kegged beer. Descriptors: shelf life of kegged beer transferred to bottles, growth of microorganisms.

It is necessary to use EBC Microbiology 2.2.1.5 and 4.3.1.1

• 26/10/2018
• 2860
• |
• Detection of Contaminants
• |
• 2011

4.3.1.4 - Enrichment

Qualitative detection of microorganisms in bottled beer. Descriptors: enrichment, growth of microorganisms.

It is necessary to use EBC Microbiology 4.3.1.1

• 26/10/2018
• 3804
• |
• Detection of Contaminants
• |
• 2011

4.3.2.1 - General Aerobic Count on Beer

Quantitative evaluation of aerobic and facultative microorganisms in filtered beer, recovered beer, beer in keg, etc. Descriptors: general aerobic count on beer, growth of microorganisms.

It is necesary to use EBC Microbiology 2.3.2.1 and 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 2914
• |
• Detection of Contaminants
• |
• 2011

4.3.2.2 - General Anaerobic Count on Beer

Quantitative evaluation of anaerobic microorganisms in filtered beer, recovered beer, beer in keg, etc. Descriptors: general anaerobic count on beer, growth of microorganisms.

It is necessary to use EBC Microbiology 2.3.2.1 and 2.3.2.2 and 2.3.4.3 or 2.3.4.4

• 26/10/2018
• 2898
• |
• Detection of Contaminants
• |
• 2011

4.3.3.1 - Lactic Acid Bacteria

Quantitative detection of bacteria belonging to the genera Lactobacillus and Pediococcus. Descriptors. lactic acid bacteria, selective culture medium, microscopy, gram differentiation, catalase test, membrane filtration, incubation.

It is necessary to use EBC Microbiology 2.3.2.1, 2.3.2.2, 2.3.4.3, 2.3.5.4, 2.3.6.1 or 2.3.6.2 and 2.3.7 

• 26/10/2018
• 2785
• |
• Detection of Contaminants
• |
• 2011

4.3.3.2 - Pectinatus and Megasphaera

Quantitative detection of strictly anaerobic Pectinatus and Megasphaera in bottled beer. Pectinatus and Megasphaera may arise as secondary contaminants when tunnel pasteurisation of the packaged product is not applied. Descriptors: anaerobic incubation, microscopy, selective medium.

It is necessary to use EBC Microbiology 2.3.7 and 2.3.6.1 and 2.3.6.2

• 26/10/2018
• 2955
• |
• Detection of Contaminants
• |
• 2011

4.3.3.3 - Selective enrichment of Pectinatus and Megasphaera

Qualitative detection of strictly anaerobic Pectinatus and Megasphaera in bottled  beer. Pectinatus and Megasphaera may arise as secondary contaminants when tunnel pasteurisation of the packaged product is not applied. Descriptors: selective enrichment, anaerobic incubation.

It is necessary to use EBC Microbiology 2.3.5.1, 2.3.5.2, 2.3.5.3 and 2.3.5.4 and 2.3.6.1 and 2.3.6.2

• 26/10/2018
• 2826
• |
• Detection of Contaminants
• |
• 2011

4.4.1 - General Aerobic Count in Water

Detection of culturable water bacteria. Descriptors: growth medium. For full details see: EN ISO 6222:2000 Enumeration of culturable micro-organisms — colony count by inoculation in a nutrient agar culture medium. 

It is necessary to use EBC Microbiology 2.3.3.1 and 2.3.4.2

• 26/10/2018
• 2957
• |
• Detection of Contaminants
• |
• 2011

4.4.2 - Escherichia coli and Coliform Bacteria

Detection and enumeration of Escherichia coli and coliform bacteria in potable water for human consumption. Because of the low selectivity, the method is unsuitable for water that has not been disinfected. Descriptors: membrane filtration, selective medium. For full details see: EN ISO 9308-1: 2000 Water quality — detection and enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method. 

• 26/10/2018
• 3103
• |
• Detection of Contaminants
• |
• 2011

4.4.3 - Enterococci

Detection and enumeration of intestinal enterococci in drinking water. Descriptors: membrane filtration, incubation. For full details see: ISO 7899-2:2000 Water quality — detection and enumeration of intestinal enterococci Part 2: Membrane filtration method. 

• 26/10/2018
• 3046
• |
• Detection of Contaminants
• |
• 2011

4.4.4 - Chromogenic/Fluorogenic Method for Escherichia coli and Coliform Bacteria

Detection and enumeration of Escherichia coli and coliform bacteria in water for human consumption. Descriptors: most probable number (MPN), chromogenic / fluorogenic method. EBC method 4.4.2 “Escherichia coli and Coliform Bacteria”, is based on EN ISO 9308-1: 2000 ‘Water quality — detection and enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method’. This method has been reported to have low selectivity and is not well suited for routine laboratory analysis of water.  The following methodology is now accepted in most countries as a national alternative to the EN ISO method.

It is necessary to use EBC Microbiology 2.2.3.4

• 26/10/2018
• 2971
• |
• Detection of Contaminants
• |
• 2011

4.4.5 - Clostridium perfringens (including spores) in water

Detection and enumeration of Clostridium perfringens (including spores) in water. Descriptors: membrane filtration, selective medium. As stated in : Council directive 98/83/EC On the quality of water intended for human consumption : Annex III Specifications for the analysis of parameters.

• 26/10/2018
• 3315
• |
• Detection of Contaminants
• |
• 2011

4.5.1 - Fusarium

The detection and confirmation of Fusarium in barley and malt. Descriptors: kernels incubation,  selective medium.