Collection of microorganisms from atmospheric air. Descriptors: air in yeast rooms, packing halls, microbiological laboratories, air and dust-borne microorganisms
Aseptic collection of bottles before the filling machine. Descriptors: cleaning control, returnable bottles, one-way (non returnable) bottles, cans for flash pasteurised beer.
It is necessary to use EBC Microbiology 2.3.2, 2.3.2.1 and 2.3.2.2
Sampling for hygiene monitoring of filling machines. This method is applicable to bottling and canning machines used in flash pasteurization or aseptic filling operations. Descriptors: collection control.
Sampling for cleaning control of crowners. Descriptors: seaming machines, flash pasteurised beer.
Rinsing for cleaning control. Descriptors: water from kegs and casks.
Aseptic collection of last rinse water for cleaning control. Descriptors: tanks, pipes
Swabbing for cleaning control. Descriptors: swab test, surface control, swab stick.
Sampling techniques allowing detection of strict anaerobic bacteria such as Pectinatus and Megasphaera. Descriptors: anaerobic sample handling.
A dilution technique for samples which are expected to contain too many microorganisms for immediate plating, i.e. more than 1,000 cfu per ml. Descriptors: dilution of samples, cell concentration, dilution series
A laboratory filtration technique for liquid samples which contain too few microorganisms for the detection by plating methods, i.e. less than 1 colony forming unit per ml. Descriptors: for water, filtered beer, soft drinks, mambrane filtration, membrane filter, pore size.
A standard technique for the quantitative detection of viable microorganisms that have been collected by membrane filtration. The present description does not include choice of growth medium etc., which should be specified in each individual case. Descriptors: filter membranes.
It is necessary to use EBC Microbiology 2.3.2.1
Gravimetric determination of sulphate with barium chloride.
It is necessary to use EBC Method 9.22
Detection of microorganisms in liquid samples containing more than one colony-forming units per ml. Descriptors: pour plate technique, plating technique, growth medium.
It is necessary to use EBC Microbiology 2.3.1
Detection of microorganisms in liquid samples containing more then ten colony-forming units per ml. Descriptors: surface spread plating technique.
It is necessary to use EBC Microbiology 2.3.1
Isolation of single colonies from a mixture of microorganisms in liquid or solid medium. Descriptors: pure culture, streaked out.
An incubation technique for the cultivation of aerobic and facultative organisms in Petri dishes. Descriptors: aerobic incubation, total counts, sterility test, purity test, cleaning control.
An incubation technique for the selective cultivation of anaerobic and microaerophilic organisms. Descriptors: anaerobic, catalysed carbon dioxide incubation, CO2, spoilage organisms, anaerobic incubator, air-tight jar with anaerobic generator.
An incubation technique for the selective cultivation of microaerophilic organisms. Descriptors: anaerobic, carbon dioxide purge incubation, Lactobacillus and Pediococcus, desiccator, anaerobic jar.
Incubation is the period between the inoculation of the culture medium and the examination of the plates. In other words: it is the period of growth from single cells to visible colonies. Descriptors: general rules for incubation of microorganisms, optimal growth condition, selective growth conditions.
Routine microscopy in a brewery laboratory. Descriptors: bright-field microscopy. adjustment of illumination (Köhler Principle), morphology of microorganisms, distinction between the main groups of yeast, moulds and bacteria, eye-piece, objective, Numerical Aperture (NA), standard microscope, Achromat, oil immersion microscopy.
It is necessary to use EBC Microbiology 2.3.6.1
Microscopy of yeast spores and detection of bacteria in yeast suspensions. Descriptors: phase contrast microscopy, phase contrast condenser, phase contrast objective, oscillation phase.
It is necessary to use EBC Microbiology 2.3.5.1
Fluorescence microscopy is applicable to stained yeast and bacteria cells and to nonbiological sediments of beer. Descriptors: fluorochromes, incident UV-light, diachromic mirror.
Detection and examination of colonies on agar plates or membrane filters. Descriptors: Stereo Microscopy, simple magnification.
Differentiation of Gram positive and Gram negative bacteria. Descriptors: Gram Staining, cell wall structure, crystal violet dye, red saffranin, gram variable, gram-positive, gram-negative
Rapid differentiation of Gram positive and Gram negative bacteria. Descriptors: KOH Method for Gram Differentiation, bacterial colonies.
Differentiation of catalase positive and catalase negative bacteria. Descriptors: Catalase Test, enzyme catalase, decompose hydrogen peroxide (H2O2) into H2O and O2.
Descriptors: ATP (adenosine triphosphate) Bioluminescence Methods, luciferin/luciferase, free ATP, luminometer (photometer), ATP content, microbial contamination, ATP bioluminescence reaction, relative light units (RLU), ATP analysis.
Rapid monitoring of the cleanliness of process surfaces. Descriptors: ATP Analysis of Swab Samples, ATP content, luciferase, D-luciferin, ATP standard solution for internal standardisation, free ATP, ATP concentration, specific luminometer, RLU values.
Rapid analysis of the cleanliness of rinse water after cleaning procedures. Descriptors: total ATP, ATP Analysis of Rinse Water, estimate the level of microbial contamination, bioluminescence method, ATPase, extracellular ATP, RLU values.
Rapid assessment of the risk for microbiological spoilage of packaged beer. Descriptors: ATP Analysis of Final Product, ATP content, free ATP, bioluminescence method, ATPase, extracellular ATP, RLU values.
