Analytica EBC methods list - page 5 - chemical-physical
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ANALYTICA EBC

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)
  • 23/10/2018
  • 5200
  • |
  • Wort
  • |
  • IM
  • 2015

8.10.1 - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

Analytica EBC - Free Amino Nitrogen in Wort by Segmented Flow Analysis
  • 23/10/2018
  • 3322
  • |
  • Wort
  • |
  • VM
  • 2011

8.10.2 - Free Amino Nitrogen in Wort by Segmented Flow Analysis

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Free amino nitrogen in wort by automated discrete analysis (IM)
  • 01/07/2021
  • 2442
  • |
  • Wort
  • |
  • IM
  • 2021

8.10.3 - Free amino nitrogen in wort by automated discrete analysis (IM)

EBC Method 8.10.3 Free Amino Nitrogen in Wort by Automated Discrete Analysis (IM) – 2021 allows for the determination of the free amino nitrogen content in wort using photometric measurement of OPA (oPhthaldialdehyde) and NAC (N-acetyl cysteine). The method is performed manually or automatically at a 340 nm wavelength at 37 °C temperature.

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The European reference Analytical Methods for breweries by EBC
Analytica EBC - pH of Wort
  • 23/10/2018
  • 4192
  • |
  • Wort
  • |
  • 1999

8.17 - pH of Wort

The determination of pH at 20 °C of wort; using a pH meter and a suitable electrode system.

It is necessary to use EBC Method 1.5

Analytica EBC - Dissolved Oxygen in Wort using Optical Sensors
  • 23/10/2018
  • 2750
  • |
  • Wort
  • |
  • 2015

8.19.1 - Dissolved Oxygen in Wort using Optical Sensors

The determination of dissolved oxygen in wort using optical oxygen sensors. Descriptors: solubility of oxygen in water in equilibrium with air at 1,01325 bar and vapour pressure of water in equilibrium with air, solubility of oxygen.

it is necessary to use EBC Method 9.37.1

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Iso-alpha-acids and alpha-acids in wort by HPLC
  • 30/09/2022
  • 982
  • |
  • Wort
  • |
  • GM
  • 2022

8.20 - Iso-alpha-acids and alpha-acids in wort by HPLC

This method specifies a high performance liquid chromatographic technique for the simultaneous determination of iso-α-acids (iso) and α-acids (alpha) in wort. It is recommended for wort samples containing α-acids and iso-α-acids. Due to the coelution of iso-α-acids (or α-acids) and reduced iso-α-acids, this method is not recommended for the analysis of wort samples containing reduced iso-α-acids.

Analytica EBC - Alcohol in Beer by Distillation
  • 23/10/2018
  • 8039
  • |
  • Beer
  • |
  • 2008

9.2.1 - Alcohol in Beer by Distillation

The determination of the alcohol content of beer using a distillation procedure and measurement of specific gravity of the distillate. Alternative procedures may produce accurate results but the distillation procedure is regarded as the reference method for alcohol.

It is necessary to use EBC Method 9.43.1 and 9.46

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Alcohol in Beer by Catalytic Combustion
  • 23/10/2018
  • 3268
  • |
  • Beer
  • |
  • AM
  • 2007

9.2.2 - Alcohol in Beer by Catalytic Combustion

The rapid determination of the alcohol content of beer using an instrument relying on catalytic combustion. The description of the method is intended only as a guide since the instrumental procedure differs from model to model.

It is necessary to use EBC Method 9.2.1

Analytica EBC - Ethanol in Beer by Gas Chromatography
  • 23/10/2018
  • 4094
  • |
  • Beer
  • |
  • 2007

9.2.4 - Ethanol in Beer by Gas Chromatography

The determination of the ethanol content of beer by gas liquid chromatography using direct liquid injection.

It is necessary to use EBC Method 9.2.1 and 9.43.1

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Analytica EBC - Alcohol in Beer by Near Infrared Spectroscopy
  • 23/10/2018
  • 4781
  • |
  • Beer
  • |
  • 2008

9.2.6 - Alcohol in Beer by Near Infrared Spectroscopy

The rapid determination of the alcohol content of beer using an automated instrument relying on near infrared spectroscopy (NIR). The description of the method is intended only as a guide since there are different types of NIR equipment available and the instrumental procedure differs from model to model.

It is necessary to use EBC Method 9.2.1 and 9.43.1 or 9.43.2

Analytica EBC - Original, Real and Apparent Extract and Original Gravity of Beer
  • 23/10/2018
  • 7183
  • |
  • Beer
  • |
  • 2004

9.4 - Original, Real and Apparent Extract and Original Gravity of Beer

The calculation of original, real and apparent extract and original gravity of beer from specific gravity determinations obtained on the beer and on the alcoholic distillate and beer residue after distillation.

It is necessary to use EBC Methods 9.2.1 and 9.43.2 or 9.43.1

The European reference Analytical Methods for breweries by EBC
Analytica EBC - Real Degree of Fermentation of Beer
  • 23/10/2018
  • 4635
  • |
  • Beer
  • |
  • 1997

9.5 - Real Degree of Fermentation of Beer

The determination of real degree of fermentation by calculation from measurements of alcohol content and real extract.

It is necessary to use EBC Methods 9.2.1 and 9.4

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