ANALYTICA EBC

• 23/10/2018
• 4245
• |
• Beer
• |
• 2000

9.7 - Final Attenuation of Beer

The determination of the final attenuation of beer using a yeast procedure.

It is necessary to use EBC Methods 8.6.1 and 8.6.2

• 23/11/2020
• 11746
• |
• Beer
• |
• IM
• 2020

9.8 - Bitterness of Beer (IM)

The determination of the bitter substances in beer, which are mainly iso-α-acids.
This Method was updated in 2020. The update covers the “Precision Chapter”. It now contains two tables. In addition to the table on conventionally-hopped beers, it also reflects the results of a second collaborative trial on dry-hopped beers carried out in 2019 covering higher BU levels.

• 23/10/2018
• 3875
• |
• Beer
• |
• 2000

9.9.1 - Total Nitrogen in Beer: Kjeldahl Method

The determination of total nitrogen content of beer by a Kjeldahl procedure.

It is necessary to use EBC Method 3.3.1

• 23/10/2018
• 3304
• |
• Beer
• |
• 1999

9.9.2 - Total Nitrogen in Beer: Dumas Combustion Method

The determination of the total nitrogen content of beer, by the Dumas combustion method.

• 23/10/2018
• 4044
• |
• Beer
• |
• IM
• 2000

9.10.1 - Free Amino Nitrogen in Beer by Spectrophotometry (IM)

The international method for the determination of free amino nitrogen in pale or dark beers. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used.

It is necessary to use EBC Method 8.10

• 23/10/2018
• 3192
• |
• Beer
• |
• 2017

9.10.2 - Free amino niytrogen in Beer by discrete analyser (NOPA Method)

The determination of the free amino nitrogen content in beer using photometric measurement of OPA (o-Phthaldialdehyde) and NAC (N-acetyl cysteine). The method is performed manually or automatically at a 340 nm wavelength at 37 °C temperature. In the automated method, the side wavelength of 700 or 750 nm is also measured. The method estimates amino acids by measuring the terminal α-amino nitrogen groups of peptides and proteins. The method is specific for α-amino nitrogen. Proline, ammonia or ammonium are not included.

• 23/10/2018
• 5500
• |
• Beer
• |
• IM
• 2002

9.11 - Total Polyphenols in Beer by Spectrophotometry (IM)

The determination of total polyphenols in beer by spectrophotometry. Descriptors: weight required, percentage of iron in the ammonium iron citrate, polyphenol content, absorbance at 600 nm, dilution factor.

• 23/10/2018
• 3525
• |
• Beer
• |
• 1997

9.12 - Flavanoids in Beer by Spectrophotometry

The determination of flavanoids in beer by spectrophotometry.

• 23/10/2018
• 3274
• |
• Beer
• |
• AM
• 2013

9.13.1 - Iron in Beer by Spectrophotometry with 2,2-Bipyridyl or 1,10-Phenanthroline

International method for the determination of iron in beer by spectrophotometry. Most of the cations of interest in brewing can be conveniently and quickly measured by atomic absorption spectrophotometry, using methods described in the literature. Descriptors: Iron in mg/litre, Calibration factor derived from the calibration graph, Absorbance at 505 or 520 nm with iron reagent added to the beer, Absorbance at 505 or 520 nm with water added to the beer.

• 23/10/2018
• 3578
• |
• Beer
• |
• AM
• 1997

9.13.2 - Iron in Beer by Spectrophotometry with Ferrozine (IM)

The determination of iron in beer by spectrophotometry using Ferrozine. Descriptors: absorbance at 562 nm for the test solution, absorbance at 562 nm for the sample blank solution, standard calibration slope.

• 23/10/2018
• 3427
• |
• Beer
• |
• IM
• 1997

9.13.3 - Iron in Beer by Atomic Absorption Spectrophotometry (IM)

International method for the determination of iron in beer by atomic absorption spectrophotometry.

• 23/10/2018
• 3118
• |
• Beer
• |
• AM
• 1997

9.14.1 - Copper in Beer by Spectrophotometry with ZDBT (IM)

International method for the determination of copper in beer by spectrophotometry.

• 23/10/2018
• 3210
• |
• Beer
• |
• AM
• 1997

9.14.2 - Copper in Beer by Spectrophotometry with Cuprethol: Rapid Method (IM)

Determination of copper in beer by the method of Brenner et al. as modified by the EBC Analysis Committee 

• 23/10/2018
• 3114
• |
• Beer
• |
• IM
• 1997

9.14.3 - Copper in Beer By Atomic Absorption Spectrophotometry (IM)

International method for the determination of copper by atomic absorption spectrophotometry.

• 23/10/2018
• 3496
• |
• Beer
• |
• AM
• 1997

9.15 - Nickel in Beer by Spectrophotometry with Dimethylglyoxime

Determination of nickel in beer by spectrophotometric measurement. Nickel can be present in beer through contact with stainless steel or Monel metal.

• 23/10/2018
• 2772
• |
• Beer
• |
• IM
• 1997

9.16 - Sodium in Beer by Atomic Absorption Spectrophotometry (IM)

International method for the determination of sodium in beer by atomic absorption spectrophotometry.

It is necessary to use EBC Method 1.3

• 23/10/2018
• 2985
• |
• Beer
• |
• IM
• 1997

9.17 - Potassium in Beer by Atomic Absorption Spectrophotometry (IM)

The determination of potassium in beer by atomic absorption spectrophotometry.

• 23/10/2018
• 3041
• |
• Beer
• |
• IM
• 1997

9.18 - Magnesium in Beer by Atomic Absorption Spectrophotometry (IM)

The determination of magnesium in beer by atomic absorption spectrophotometry.

It is necessary to use EBC Method 1.3

• 23/10/2018
• 3317
• |
• Beer
• |
• IM
• 2002

9.19 - Calcium in Beer by Atomic Absorption Spectrophotometry (IM)

International Method for the determination of calcium in beer by atomic absorption spectrophotometry.

It is necessary to use EBC Method 8.18

• 23/10/2018
• 3009
• |
• Beer
• |
• IM
• 1997

9.20 - Zinc in Beer by Atomic Absorption Spectrophotometry (IM)

The determination of zinc in beer by atomic absorption spectrophotometry. Descriptors: example of the calibration of the level of zinc obtained by atomic absorption spectrophotometry

• 23/10/2018
• 3447
• |
• Beer
• |
• AM
• 1996

9.21 - Chloride in Beer: Conductometric Method (IM)

Conductometric method for the determination of chloride in beer, by precipitation as silver chloride. 

• 23/10/2018
• 3066
• |
• Beer
• |
• AM
• 1997

9.22 - Sulphate in Beer: Gravimetric Method

• 23/10/2018
• 3115
• |
• Beer
• |
• AM
• 1997

9.23 - Nitrate in Beer: Enzymatic Method

The determination of nitrate in beer using an enzymatic procedure. 

• 23/10/2018
• 4461
• |
• Beer
• |
• 2000

9.24.1 - Vicinal Diketones in Beer: Spectrophotometric Method

The determination of vicinal diketones in beer by ultraviolet spectrophotometry.

It is necessary to use EBC Method 9.24.2

• 23/10/2018
• 4032
• |
• Beer
• |
• 1999

9.24.2 - Vicinal Diketones in Beer: Gas Chromatographic Method

Determination of the vicinal diketones, 2,3-butanedione (diacetyl), 2,3-­pentanedione and their precursors in fermenting worts and beers by gas chromatography using a capillary column.

• 23/10/2018
• 3767
• |
• Beer
• |
• 1997

9.25.1 - Total Sulphur Dioxide in Beer: Distillation Method

The determination of sulphur dioxide by distillation and titration.

• 23/10/2018
• 2792
• |
• Beer
• |
• AM
• 1997

9.25.2 - Total Sulphur Dioxide in Beer: Enzymatic Method

The determination of sulphur dioxide in beer, wort and water using an enzymatic procedure. 

• 23/10/2018
• 4147
• |
• Beer
• |
• IM
• 2005

9.25.3 - Total Sulphur Dioxide in Beer: p-Rosaniline Method (IM)

The determination of total sulphur dioxide in beer using colorimetry with p-rosaniline.

• 23/10/2018
• 3080
• |
• Beer
• |
• 2000

9.26 - Total Carbohydrate in Beer by Spectrophotometry

The determination of the total carbohydrate content of beer by spectrophotometry.

• 23/10/2018
• 3400
• |
• Beer
• |
• IM
• 1997

9.27 - Fermentable Carbohydrates in Beer by HPLC (IM)

The determination of fructose, glucose and sugars with 2 and 3 degrees of polymerization (DP2 and DP3) by high performance liquid chromatography (HPLC).

It is necessary to use EBC Method 8.7