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ANALYTICA EBC

Analytica EBC - Free Amino Nitrogen in Wort by Segmented Flow Analysis (IM)

8.10.2 - Free Amino Nitrogen in Wort by Segmented Flow Analysis (IM)

  • 23/10/2018
  • 247
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  • Wort
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  • IM

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.