The method is used to obtain a count of potential beer spoilage organisms in dilute sugars when a low level of contamination is expected. Microorganisms are concentrated by membrane filtration. Descriptors: general aerobic count in dilute sugars, nutrient medium.
It is necessary to use EBC Microbiology 2.3.2.1
To assess the microbiological quality of any process gas. This method is used to obtain a total count when small numbers of microorganisms are expected. Descriptors: general aerobic count in process gases, nutrient medium, CFU, membrane filtration.
It is necessary to use EBC Microbiology 2.2.4.1 and 2.3.2.1
Quantitative evaluation of aerobic and facultative microorganisms in caustic or acid solutions of CIP recovery systems. Both acid and caustic recovery systems can become contaminated and it is recommended that tanks used to recover acid or caustic should be regularly assessed for this reason. Descriptors: general aerobic count in acid and caustic recovery systems.
The efficacy of chemical disinfectants should preferably be tested according to the approved methods in the guidelines of the European Committee for Standardisation (CEN). Descriptors: efficacy of disinfectants, bovine albumin, standardised hard water, European standards for testing chemical disinfectants, microbiological evaluation of the efficacy of sterilants and biocides.
Detection of Saccharomyces cerevisiae var. diastaticus in samples of brewers' yeast, fermenting, filtered and packaged beer and the production environment including water, swabs and air.
Descriptors: Saccharomyces cerevisiae var. diastaticus, super attenuation, over carbonation, spoilage, wild yeast, PCR.
The following list contains details of inhibitors that have been reported to be valuable in the selective growth of brewery organisms. Some ready-to-use media contain inhibitors included by the manufacturer. Others are base media where inhibitors must be added with due regard to heat sensitivity. Workers should pay careful attention to the safety and hazard information supplied by the manufacturer when handling these chemicals.
Preparation of Growth Media.
A list of dehydrated and liquid media.
Recipes of a number of selected media are given below. Full descriptions of commercially available media are normally given on the package.
List and recipe of reagents in brewery microbiology
EU Regulation on hygiene of foodstuffs, quality of water and ISO/EN norms on water analysis as wella s rules applying outside the European Union.