Analytica EBC methods list - page 2 - microbiology
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ANALYTICA EBC

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2.3.1 - Dilution of Samples

A dilution technique for samples which are expected to contain too many microorganisms for immediate plating, i.e. more than 1,000 cfu per ml. Descriptors: dilution of samples, cell concentration, dilution series

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2.3.2.1 - Laboratory Membrane Filtration Technique

A laboratory filtration technique for liquid samples which contain too few microorganisms for the detection by plating methods, i.e. less than 1 colony forming unit per ml. Descriptors: for water, filtered beer, soft drinks, mambrane filtration, membrane filter, pore size.

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2.3.2.2 - Filter Membrane Growth Technique

A standard technique for the quantitative detection of viable microorganisms that have been collected by membrane filtration. The present description does not include choice of growth medium etc., which should be specified in each individual case. Descriptors: filter membranes.

It is necessary to use EBC Microbiology 2.3.2.1

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2.3.3.1 - Pour Plate Technique

Detection of microorganisms in liquid samples containing more than one colony-forming units per ml. Descriptors: pour plate technique, plating technique, growth medium.

It is necessary to use EBC Microbiology 2.3.1

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2.3.3.2 - Surface Spread Plating Technique

Detection of microorganisms in liquid samples containing more then ten colony-forming units per ml. Descriptors: surface spread plating technique.

It is necessary to use EBC Microbiology 2.3.1

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2.3.3.3 - Isolation of Single Colonies

Isolation of single colonies from a mixture of microorganisms in liquid or solid medium. Descriptors:  pure culture, streaked out.

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2.3.4.2 - Aerobic Incubation

An incubation technique for the cultivation of aerobic and facultative organisms in Petri dishes. Descriptors: aerobic incubation, total counts,  sterility test, purity test, cleaning control.

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2.3.4.3 - Anaerobic, Catalysed Carbon Dioxide Incubation

An incubation technique for the selective cultivation of anaerobic and microaerophilic organisms. Descriptors: anaerobic, catalysed carbon dioxide incubation, CO2, spoilage organisms, anaerobic incubator, air-tight jar with anaerobic generator.

The European reference Analytical Methods for breweries by EBC
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2.3.4.4 - Anaerobic, Carbon Dioxide Purge Incubation

An incubation technique for the selective cultivation of microaerophilic organisms. Descriptors: anaerobic, carbon dioxide purge incubation, Lactobacillus and Pediococcus, desiccator, anaerobic jar.

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2.3.4.1 - General Rules for Incubation

Incubation is the period between the inoculation of the culture medium and the examination of the plates. In other words: it is the period of growth from single cells to visible colonies. Descriptors: general rules for incubation of microorganisms,  optimal growth condition, selective growth conditions.

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2.3.5.1 - Bright Field Microscopy

Routine microscopy in a brewery laboratory. Descriptors: bright-field microscopy. adjustment of illumination (Köhler Principle), morphology of microorganisms, distinction between the main groups of yeast, moulds and bacteria, eye-piece, objective, Numerical Aperture (NA), standard microscope, Achromat, oil immersion microscopy.

It is necessary to use EBC Microbiology 2.3.6.1

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2.3.5.2 - Phase Contrast Microscopy

Microscopy of yeast spores and detection of bacteria in yeast suspensions. Descriptors: phase contrast microscopy, phase contrast condenser, phase contrast objective, oscillation phase.

It is necessary to use EBC Microbiology 2.3.5.1

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2.3.5.3 - Fluorescence Microscopy

Fluorescence microscopy is applicable to stained yeast and bacteria cells and to nonbiological sediments of beer. Descriptors: fluorochromes,  incident UV-light,  diachromic mirror.

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2.3.5.4 - Stereo Microscopy

Detection and examination of colonies on agar plates or membrane filters. Descriptors: Stereo Microscopy, simple magnification.

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2.3.6.1 - Gram Staining

Differentiation of Gram positive and Gram negative bacteria. Descriptors: Gram Staining, cell wall structure, crystal violet dye, red saffranin, gram variable, gram-positive, gram-negative

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2.3.6.2 - KOH Method for Gram Differentiation

Rapid differentiation of Gram positive and Gram negative bacteria. Descriptors: KOH Method for Gram Differentiation, bacterial colonies.

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2.3.7 - Catalase Test

Differentiation of catalase positive and catalase negative bacteria. Descriptors: Catalase Test, enzyme catalase, decompose hydrogen peroxide (H2O2) into H2O and O2.

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2.3.8 - ATP Bioluminescence Methods

Descriptors: ATP (adenosine triphosphate) Bioluminescence Methods, luciferin/luciferase, free ATP, luminometer (photometer), ATP content, microbial contamination, ATP bioluminescence reaction, relative light units (RLU), ATP analysis.

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2.3.8.1 - ATP Analysis of Swab Samples

Rapid monitoring of the cleanliness of process surfaces. Descriptors: ATP Analysis of Swab Samples, ATP content, luciferase, D-luciferin, ATP standard solution for internal standardisation, free ATP, ATP concentration, specific luminometer, RLU values.

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2.3.8.2 - ATP Analysis of Rinse Water

Rapid analysis of the cleanliness of rinse water after cleaning procedures. Descriptors: total ATP, ATP Analysis of Rinse Water, estimate the level of microbial contamination, bioluminescence method,  ATPase, extracellular ATP, RLU values.

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2.3.8.3 - ATP Analysis of Final Product

Rapid assessment of the risk for microbiological spoilage of packaged beer. Descriptors: ATP Analysis of Final Product, ATP content, free ATP, bioluminescence method,  ATPase, extracellular ATP, RLU values.

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2.3.9.1 - Gram Staining for Differentiation of Bacteria

Differentiation of Gram positive and Gram negative bacteria. Descriptors: Gram Staining for Differentiation of Bacteria,  cell wall structure, crystal violet dye, red saffranin.

  • 25/10/2018
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  • 2011

2.3.9.3 - Catalase Test

Differentiation of catalase positive and catalase negative bacteria. Descriptors: Catalase Test, enzyme catalase, decompose hydrogen peroxide (H2O2) into H2O and O2.

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2.3.9.4 - Oxidase Test

Differentiation of oxidase positive and oxidase negative bacteria. Descriptors: Oxidase Test, tetramethyl-p-phenylene diamine, enzyme oxidase.

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2.3.9.5 - Phenolic Off Flavour (POF)

Yeasts isolated on agar plates can be checked for spoilage potential by their ability to decarboxylate an organic acid, producing a distinctive odour. This method, originally described by EBC, was adopted by the ASBC as an international method in 2016 / 2017 and reciprocated as an international method by EBC under the same number in 2018. Descriptors: Phenolic Off Flavour (POF), decarboxylate ferulic acid to 4-vinyl guaiacol, aroma of cloves.

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2.4.1.1 - Detection and Identification of Microorganisms from Turbid Liquid Samples or Cultured Colonies by Real-Time PCR (Polymerase Chain Reaction)

Analysis of turbid samples (enrichments or yeast containing) and of colonies from agar plates for the presence and identification of bacteria, yeasts and/or moulds by detection of their DNA (deoxyribonucleic acid). Descriptors: Detection and Identification of Microorganisms from Turbid Liquid.

It is necessary to use EBC Microbiology 2.3.9.1 to 2.3.9.5

The European reference Analytical Methods for breweries by EBC
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2.4.1.2 - Detection and Identification of Microorganisms from Filterable Samples by Real-Time PCR (Polymerase Chain Reaction)

Analysis of filterable samples for the presence and identification of bacteria, yeasts and/or moulds by detection of their DNA (deoxyribonucleic acid). Descriptors: Detection and Identification of Microorganisms from Filterable Samples by Real-Time PCR (Polymerase Chain Reaction), PCR analysis, DNA Extraction, PCR thermocycling instrument, specific primers and probes systems in PCR, lysis buffer, DNA polymerase (usually TaqPolymerase), RealTime PCR thermocycling instrument,  Real Time PCR, ready-to-use DNA extraction kits and PCR applications.

It is necessary to use EBC Microbiology 2.3.2.1 and 2.3.9.1 to 2.3.9.5