Analytica EBC methods list - page 2 - chemical-physical - beer
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ANALYTICA EBC

Analytica EBC - Total sulphur dioxide in beer by DTNB method with quick test kits (IM)
  • 29/06/2026
  • 28
  • |
  • Beer
  • |
  • 2024

9.25.5 - Total sulphur dioxide in beer by DTNB method with quick test kits (IM)

The purpose of this method is the determination of total sulphur dioxide in beer. Sulphites, like sulphur dioxide, are a group of sulphur-based substances which are produced naturally during fermentation and are also used as food additives for their preservative and antioxidant properties. This method describes the determination of Total Sulphur Dioxide in beer using DTNB method with quick test kits a) CDR BeerLab®, b) Merck Spectroquant, c) Megazyme K-TSULPH of Neogen. The method is based on the reaction between sulphur dioxide and 5,5’-dithiobis nitrobenzoic acid (DTNB) in basic conditions. The absorption is measured on a photometer of the supplier (CDR BeerLab®) or a spectrophotometer (Spectroquant or Megazyme test). The method has been validated to cover a total sulphur dioxide range of 1,0 to 19,4 mg/L.  Basic laboratory skills, including pipetting skills, are sufficient to accomplish the semi-automated (CDR BeerLab®) or manual (Spectroquant/Megazyme) method successfully, the beer samples should be briefly degassed. Labour time and total analysis time are short; within 1 hour (CDR BeerLab®/Megazyme) the results can be reported of a few samples; for Spectroquant you need 1 to 2 hours. The reagents used do not present a specific risk, however you need to wear safety gloves and glasses when you work with reagent 1 (SO3-1) of Megazyme.

Analytica EBC - Fermentable Carbohydrates in Beer by HPLC (IM)
  • 23/10/2018
  • 4551
  • |
  • Beer
  • |
  • IM
  • 1997

9.27 - Fermentable Carbohydrates in Beer by HPLC (IM)

The determination of fructose, glucose and sugars with 2 and 3 degrees of polymerization (DP2 and DP3) by high performance liquid chromatography (HPLC).

It is necessary to use EBC Method 8.7

Analytica EBC - Carbon Dioxide in Beer: Titration Method (RM)
  • 23/10/2018
  • 4487
  • |
  • Beer
  • |
  • GM
  • 1997

9.28.1 - Carbon Dioxide in Beer: Titration Method (RM)

The determination of carbon dioxide in beers (or in other carbonated beverages) using the Blom method which relies on titration. Descriptors: apparatus for determination of carbon dioxide by titration method (blom)

Analytica EBC - Carbon Dioxide in Beer: Instrumental Method
  • 23/10/2018
  • 3811
  • |
  • Beer
  • |
  • AM
  • 1997

9.28.2 - Carbon Dioxide in Beer: Instrumental Method

The determination of carbon dioxide in beer (or in other carbonated beverages) by an instrumental procedure using a Mettler Toledo (formerly Corning) 965D carbon dioxide analyser. 

Analytica EBC - Carbon Dioxide in Beer: Pressure Method
  • 23/10/2018
  • 5790
  • |
  • Beer
  • |
  • 2007

9.28.3 - Carbon Dioxide in Beer: Pressure Method

The determination of the dissolved carbon dioxide concentration in small package (PET/glass bottles and cans) beer by a pressure method. The description of the method is intended as a guide as there is such a diversity of instruments in use.

It is necessary to use EBC Method 9.28.1 and 11.2.2

Analytica EBC - Measurement of Dissolved Oxygen by Optical Sensors
  • 23/10/2018
  • 4177
  • |
  • Beer
  • |
  • 2015

9.37.1 - Measurement of Dissolved Oxygen by Optical Sensors

The determination of dissolved oxygen in liquor, wort and beer by optical oxygen sensors. Descriptors: solubility of oxygen in water in equilibrium with air at 1,01325 bar and vapour pressure of water in equilibrium with air, solubility of oxygen

Analytica EBC

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