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Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

  • 23/10/2018

Free Amino Nitrogen in Wort by Segmented Flow Analysis

The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.

  • 23/10/2018

Sampling of Hops and Hop Products

Descriptors: sampling of hops an hop products, unpacked hops, packed hops, hop powder and pellets, isomerised hop products, hop extract.

It is necessary to use EBC Method 2.1

  • 23/10/2018

Moisture Content of Hops and Hop Products

The determination of the moisture content of hops, hop powders and hop pellets by loss on drying. Descriptors: calculate the moisture percentage of the sample.

 

  • 23/10/2018

Seed Content of Hops

Determination of the seed content of whole hops by separation of the remaining material. Descriptors: calculate the seed content.

  • 23/10/2018

Lead Conductance Value of Hops, Powders and Pellets

The determination of the lead conductance value of hops, hop powders and hop pellets by conductometry. Descriptors: concentration of lead acetate, calculate the lead conductance value of hops.

This Method was last updated in January 2019. The updates cover the “Procedure” and “Precision” chapters. In the “Procedure” chapter, the extraction parameters are more specified and the method of conductometric titration has been harmonized with Method 7.5 “Bitter Substances in Hops and Hop Products: Lead Conductance Value and Total Resin, Soft Resin and Hard Resin”. The “Precision” chapter now reflects the recent results of a collaborative trial on both hops and pellets carried out in 2018 covering a broader range from low to high alpha varieties as typically used today.

It is necessary to use EBC Method 7.1

  • 23/01/2019

α- and β-Acids in Hops and Hop Products by HPLC7

The method specifies a high performance liquid chromatographic analysis for the determination of α- and β-acids for all hops, hop powder products as well as for all conventional hop extracts.

It is necessary to use EBC Methods 7.1 and 7.6.

 

 

  • 23/10/2018

Iso-α-Acids and reduced iso-α-Acids in Hop Products by HPLC

This method specifies an isocratic high performance liquid chromatographic method for the determination of all types of iso-α-acids and reduced iso-α-acids (but not mixtures of different types) in hop products. Descriptors: concentration of a particular type of iso-α-acids, response factor for a particular type of iso-α-acids.

It is necessary to use EBC Methods 7.1, 7.7, 7.6 and 7.8.

  • 23/10/2018

Hop Oil Content of Hops and Hop Products

The determination of the oil content of hops, hop powders, hop pellets and hop extracts by steam distillation. Descriptors: institute of brewing still.

It is necessary to use EBC Method 7.1

  • 23/10/2018

Hop Storage Index of Hops and Hop Pellets

The method specifies a spectrophotometric procedure for the determination of the Hop Storage Index (HSI) of hops or hop pellets. The HSI is variety-dependent to some degree and measures an oxidative decrease in both α- and β-acids.

It is necessary to use EBC Methods 1.2 and 7.1.

  • 23/10/2018

Xanthohumol in Hops and Hop Products by HPLC

The method specifies a high-performance liquid chromatographic analysis method for the determination of xanthohumol for all hops, hop powder products as well as for all conventional or xanthohumol-rich hop extracts.

It is necessary to use EBC Methods 7.1, 7.7 and 7.8

  • 23/10/2018

Sampling of Wort

This method describes procedures employed to obtain homogeneous and representative samples of wort for analysis.

  • 23/10/2018

Specific Gravity of Wort using a Pyknometer

The determination of the specific gravity and the defined specific gravity/density derived values of wort in air at 20 °C by weighing a known volume in a pyknometer or gravity bottle. Descriptors: pyknometer, reischauer type, gay-lussac type, gravity bottle.

  • 23/10/2018