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Pour Plate Technique

Detection of microorganisms in liquid samples containing more than one colony-forming units per ml. Descriptors: pour plate technique, plating technique, growth medium.

It is necessary to use EBC Microbiology 2.3.1

  • 25/10/2018

Method Selection Guide

This Guide lists the sampling methods, analysis techniques and confirmatory tests which are associated with the following topical areas: 1) brewing materials - Barley & Malt; 2) Brewing Materials: Additives and Adjuncts; 3) Brewing Materials: Process Gases; 4) Brewing Materials: Brewers' grains; 5) Brewing Materials: Water; 6) Wort; 7) Yeast: Storage, Supply and Propagation; 8) Yeast: Physiology and Characterisation 9) Yeast: Propagation and Yeast during fermentation; 10) Yeast: Detection of Contaminants; 11) Beer in process: samples containing yeasts (fermentation, green beer, cask beer, etc) - Detection of contaminants; 12) Beer in process: bright beer and recovered beer - detection of contaminants; 13) Beer in Package: keg and cask; 14) Beer in Package: can and bottle; 15) Brewery Hygiene Monitoring

  • 25/10/2018

Principles of Microbiology

This document lays down the principles of microbiology with an emphasis on beer-related microbiology. It contains the following chapters and sub-chapters: 1) Principles of Microbiology; 2) Brewery microbiology; 2.1) Classification of microorganisms; 2.2) The microflora of barley, malt and beer; 3) Controlling Brewery Hygiene; 4) Yeast Microbiology; 4.1) Geenral description of yeasts; 4.2) Brewers' yeast: saccharomyces pastorianus and saccharomyces cerevisia; 4.3) wild yeasts; 5) Bacteria; 5.1) General description of bacteria; 5.2) Gram-positive spoilage bacteria; 5.3) Gram-negative spoilage bacteria; 6) Moulds; 7) Literature

  • 25/10/2018

Sampling by Valves

Aseptic sampling from tanks and pipelines through valve type samplers.

  • 25/10/2018

On-Line Sampling by Membrane Filtration

On line membrane filtration to collect oxygen tolerant microorganisms from a standardised, limited volume or through a bypass for a certain time. Descriptors: filterable liquid samples, membrane filter, pore size

  • 25/10/2018

Counterpressure Sampling

Aseptic sampling without losing CO2. Descriptors: Counterpressure Sampling, shelf life of bulk beer.

It is necessary to use EBC Microbiology 2.2.1.2

  • 25/10/2018

Sampling for Yeast Cell Counts

Collection of samples for the determination of the number of brewers' yeast cells (i.e. for an analysis of physical rather than microbiological characteristics). Descriptors: Sampling for Yeast Cell Counts, Copper sulphate

  • 25/10/2018

Sampling of Thick Yeast from Closed Vessels

Aseptic sampling of thick yeast from a tank. Descriptors: sampling of thick pitching yeast from closed vessels, diaphragm, valve, sample cock.

It is necessary to use EBC Microbiology 2.2.1.2, 2.2.1.3 and 2.2.1.1

  • 25/10/2018

Autoclaving

A laboratory method for sterilisation of materials which can withstand moist heat. Descriptors: autoclaving, autoclave

  • 24/10/2018

Intermittent Sterilisation (Tyndallisation)

A laboratory method for sterilisation of media with constituents which will decompose if exposed to temperatures above 100 °C. Descriptors: intermittent sterilisation (tyndallisation), bacterial spores, steam chamber.

It is necessary to use EBC Microbiology 2.1.2

  • 24/10/2018

Pasteurisation

A general method for stabilising fluids like media, beer or wort which should not be exposed to temperatures above 100 °C in tightly closed vessels. Descriptors: pasteurisation, kill microorganisms by heat, Pasteurisation Units, tunnel pasteuriser for bottled beer, PU.

It is necessary to use EBC Microbiology 2.1.2

  • 24/10/2018

Sterile Filtration

A laboratory method for the sterilisation of large volumes of heat sensitive liquids. The liquid must not contain particles which block the pores of the membrane. Descriptors: sterile filtration, removal of microorganisms, pore size, pressure filtration, vacuum filtration.

  • 24/10/2018

Sampling by Diaphragm Samplers

Aseptic sampling from tanks or pipelines through rubber diaphragms. Descriptors: hypodermic needle, safest of all methods with regard to aseptic sampling, sample fitting.

  • 24/10/2018

Dry Heat Sterilisation

A laboratory method for the sterilisation of utensils which should be kept dry and are resistant to high temperature. Descriptors: dry heat sterilisation, hot dry air

  • 24/10/2018