Routine microscopy in a brewery laboratory. Descriptors: bright-field microscopy. adjustment of illumination (Köhler Principle), morphology of microorganisms, distinction between the main groups of yeast, moulds and bacteria, eye-piece, objective, Numerical Aperture (NA), standard microscope, Achromat, oil immersion microscopy.
It is necessary to use EBC Microbiology 2.3.6.1
Microscopy of yeast spores and detection of bacteria in yeast suspensions. Descriptors: phase contrast microscopy, phase contrast condenser, phase contrast objective, oscillation phase.
It is necessary to use EBC Microbiology 2.3.5.1
Fluorescence microscopy is applicable to stained yeast and bacteria cells and to nonbiological sediments of beer. Descriptors: fluorochromes, incident UV-light, diachromic mirror.
Detection and examination of colonies on agar plates or membrane filters. Descriptors: Stereo Microscopy, simple magnification.
Differentiation of Gram positive and Gram negative bacteria. Descriptors: Gram Staining, cell wall structure, crystal violet dye, red saffranin, gram variable, gram-positive, gram-negative
Rapid differentiation of Gram positive and Gram negative bacteria. Descriptors: KOH Method for Gram Differentiation, bacterial colonies.
Differentiation of catalase positive and catalase negative bacteria. Descriptors: Catalase Test, enzyme catalase, decompose hydrogen peroxide (H2O2) into H2O and O2.
Descriptors: ATP (adenosine triphosphate) Bioluminescence Methods, luciferin/luciferase, free ATP, luminometer (photometer), ATP content, microbial contamination, ATP bioluminescence reaction, relative light units (RLU), ATP analysis.
Rapid monitoring of the cleanliness of process surfaces. Descriptors: ATP Analysis of Swab Samples, ATP content, luciferase, D-luciferin, ATP standard solution for internal standardisation, free ATP, ATP concentration, specific luminometer, RLU values.
Rapid analysis of the cleanliness of rinse water after cleaning procedures. Descriptors: total ATP, ATP Analysis of Rinse Water, estimate the level of microbial contamination, bioluminescence method, ATPase, extracellular ATP, RLU values.
Rapid assessment of the risk for microbiological spoilage of packaged beer. Descriptors: ATP Analysis of Final Product, ATP content, free ATP, bioluminescence method, ATPase, extracellular ATP, RLU values.
Aseptic sampling from large tanks which are accessible only from the top. Descriptors: raw water, open water reservoirs
Preparation of sampling flasks for neutralisation of chlorine. Descriptors. chlorinated raw water, chlorine disinfectants, sodium thiosulphate
Aseptic collection of microorganisms from process gases. Descriptors. sampling of contaminants from compressed gases, carbon dioxide, nitrogen, air, "bubble" bottle.
It is necessary to use EBC Microbiology 4.6.3
Collection of microorganisms from atmospheric air. Descriptors: air in yeast rooms, packing halls, microbiological laboratories, air and dust-borne microorganisms
Aseptic collection of bottles before the filling machine. Descriptors: cleaning control, returnable bottles, one-way (non returnable) bottles, cans for flash pasteurised beer.
It is necessary to use EBC Microbiology 2.3.2, 2.3.2.1 and 2.3.2.2
Sampling for hygiene monitoring of filling machines. This method is applicable to bottling and canning machines used in flash pasteurization or aseptic filling operations. Descriptors: collection control.
Sampling for cleaning control of crowners. Descriptors: seaming machines, flash pasteurised beer.
Rinsing for cleaning control. Descriptors: water from kegs and casks.
Aseptic collection of last rinse water for cleaning control. Descriptors: tanks, pipes
