The determination of the ethanol content of alcohol free and low alcohol beers by gas liquid chromatography using headspace injection and flame ionization detection.

The calculation of original, real and apparent extract and original gravity of beer from specific gravity determinations obtained on the beer and on the alcoholic distillate and beer residue after distillation.

It is necessary to use EBC Methods 9.2.1 and 9.43.2 or 9.43.1

The determination of real degree of fermentation by calculation from measurements of alcohol content and real extract.

It is necessary to use EBC Methods 9.2.1 and 9.4

The determination of the colour of beer by spectrophotometry.

It is necessary to use EBC Method 8.5

The determination of the final attenuation of beer using a yeast procedure.

It is necessary to use EBC Methods 8.6.1 and 8.6.2

The determination of the bitter substances in beer, which are mainly iso-α-acids.
This Method was updated in 2020. The update covers the “Precision Chapter”. It now contains two tables. In addition to the table on conventionally-hopped beers, it also reflects the results of a second collaborative trial on dry-hopped beers carried out in 2019 covering higher BU levels.

The determination of total nitrogen content of beer by a Kjeldahl procedure.

It is necessary to use EBC Method 3.3.1

The determination of the total nitrogen content of beer, by the Dumas combustion method.

The international method for the determination of free amino nitrogen in pale or dark beers. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used.

It is necessary to use EBC Method 8.10

The determination of the free amino nitrogen content in beer using photometric measurement of OPA (o-Phthaldialdehyde) and NAC (N-acetyl cysteine). The method is performed manually or automatically at a 340 nm wavelength at 37 °C temperature. In the automated method, the side wavelength of 700 or 750 nm is also measured. The method estimates amino acids by measuring the terminal α-amino nitrogen groups of peptides and proteins. The method is specific for α-amino nitrogen. Proline, ammonia or ammonium are not included.

The determination of total polyphenols in beer by spectrophotometry. Descriptors: weight required, percentage of iron in the ammonium iron citrate, polyphenol content, absorbance at 600 nm, dilution factor.

The determination of zinc in wort by atomic absorption spectrophotometry. Descriptors: calculation of the level of zinc obtained by atomic absorption spectrophotometry

The determination of total polyphenols in wort by spectrophotometry.

It is necessary to use EBC Method 9.11

Determination of the high molecular weight mixed linkage (1,3) (1,4) ß-D glucan (ß-glucan) fraction present in wort. 

It is necessary to use EBC Method 3.10.1

Determination of the high molecular weight (1,3)(1,4) β-D glucan (β-glucan) fraction present in wort.

It is necessary to use EBC Method 3.10.2

The determination of the total carbohydrate content of wort by spectrophotometry. 

Conductometric method for the determination of chloride in wort by precipitation as silver chloride. 

It is necessary to use EBC Method 9.21

Gravimetric determination of sulphate with barium chloride. 

It is necessary to use EBC Method 9.22

The determination of pH at 20 °C of wort; using a pH meter and a suitable electrode system.

It is necessary to use EBC Method 1.5

The determination of calcium in wort by atomic absorption spectrophotometry.

It is necessary to use EBC Method 1.3