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ANALYTICA EBC

  • 25/10/2018
  • 37
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  • Method Selection Guide
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  • 2011

0 - Method Selection Guide

This Guide lists the sampling methods, analysis techniques and confirmatory tests which are associated with the following topical areas: 1) brewing materials - Barley & Malt; 2) Brewing Materials: Additives and Adjuncts; 3) Brewing Materials: Process Gases; 4) Brewing Materials: Brewers' grains; 5) Brewing Materials: Water; 6) Wort; 7) Yeast: Storage, Supply and Propagation; 8) Yeast: Physiology and Characterisation 9) Yeast: Propagation and Yeast during fermentation; 10) Yeast: Detection of Contaminants; 11) Beer in process: samples containing yeasts (fermentation, green beer, cask beer, etc) - Detection of contaminants; 12) Beer in process: bright beer and recovered beer - detection of contaminants; 13) Beer in Package: keg and cask; 14) Beer in Package: can and bottle; 15) Brewery Hygiene Monitoring

  • 25/10/2018
  • 32
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  • Microbiology
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  • 2011

1 - Principles of Microbiology

This document lays down the principles of microbiology with an emphasis on beer-related microbiology. It contains the following chapters and sub-chapters: 1) Principles of Microbiology; 2) Brewery microbiology; 2.1) Classification of microorganisms; 2.2) The microflora of barley, malt and beer; 3) Controlling Brewery Hygiene; 4) Yeast Microbiology; 4.1) Geenral description of yeasts; 4.2) Brewers' yeast: saccharomyces pastorianus and saccharomyces cerevisia; 4.3) wild yeasts; 5) Bacteria; 5.1) General description of bacteria; 5.2) Gram-positive spoilage bacteria; 5.3) Gram-negative spoilage bacteria; 6) Moulds; 7) Literature

  • 19/10/2018
  • 23
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  • Apparatus
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  • AM
  • 1997

1.2 - Care and Adjustment of Apparatus: Spectrophotometers

Numerous spectrophotometric measurements are employed in brewing. To obtain reliable results, it is necessary to take certain precautions. The method can be applied to the care of optical glass and silica cells, checking the wavelength and precision of the spectrophotometer.

  • 24/10/2018
  • 23
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  • Packaging and Packaging Materials
  • |
  • 2006

1.18 - Overflow Capacity of Cans

The determination of the overflow capacity of empty cans. Descriptors: Correction factors for nominal content for temperature, density of water at test temperature, volume of water, temperature of water at dispensing, apparatus for determining overflow capacity of cans.

  • 19/10/2018
  • 34
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  • Water
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  • 1997

2.1 - Sampling of water

This document deals with methods of sampling water for chemical analysis, but not for bacteriological tests. Descriptors: by-pass period sample collecor, immersion sample collector, siphon period sample collector, immersion flushing sample collector, immersion controlled sample collector, suitable materials for containers, comparative properties of plastics.

  • 24/10/2018
  • 28
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  • Microbiological Techniques
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  • 2011

2.1.1 - Dry Heat Sterilisation

A laboratory method for the sterilisation of utensils which should be kept dry and are resistant to high temperature. Descriptors: dry heat sterilisation, hot dry air

  • 24/10/2018
  • 24
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  • Microbiological Techniques
  • |
  • 2011

2.1.2 - Autoclaving

A laboratory method for sterilisation of materials which can withstand moist heat. Descriptors: autoclaving, autoclave

  • 24/10/2018
  • 23
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  • Microbiological Techniques
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  • 2011

2.1.3 - Intermittent Sterilisation (Tyndallisation)

A laboratory method for sterilisation of media with constituents which will decompose if exposed to temperatures above 100 °C. Descriptors: intermittent sterilisation (tyndallisation), bacterial spores, steam chamber.

It is necessary to use EBC Microbiology 2.1.2

  • 24/10/2018
  • 23
  • |
  • Microbiological Techniques
  • |
  • 2011

2.1.4 - Pasteurisation

A general method for stabilising fluids like media, beer or wort which should not be exposed to temperatures above 100 °C in tightly closed vessels. Descriptors: pasteurisation, kill microorganisms by heat, Pasteurisation Units, tunnel pasteuriser for bottled beer, PU.

It is necessary to use EBC Microbiology 2.1.2

  • 24/10/2018
  • 23
  • |
  • Microbiological Techniques
  • |
  • 2011

2.1.5 - Sterile Filtration

A laboratory method for the sterilisation of large volumes of heat sensitive liquids. The liquid must not contain particles which block the pores of the membrane. Descriptors: sterile filtration, removal of microorganisms, pore size, pressure filtration, vacuum filtration.

  • 24/10/2018
  • 28
  • |
  • Microbiological Techniques
  • |
  • 2011

2.2.1.1 - Sampling by Diaphragm Samplers

Aseptic sampling from tanks or pipelines through rubber diaphragms. Descriptors: hypodermic needle, safest of all methods with regard to aseptic sampling, sample fitting.

  • 25/10/2018
  • 23
  • |
  • Microbiological Techniques
  • |
  • 2011

2.2.1.3 - Sampling by Valves

Aseptic sampling from tanks and pipelines through valve type samplers.

  • 25/10/2018
  • 23
  • |
  • Microbiological Techniques
  • |
  • 2011

2.2.1.4 - On-Line Sampling by Membrane Filtration

On line membrane filtration to collect oxygen tolerant microorganisms from a standardised, limited volume or through a bypass for a certain time. Descriptors: filterable liquid samples, membrane filter, pore size

  • 25/10/2018
  • 23
  • |
  • Microbiological Techniques
  • |
  • 2011

2.2.1.5 - Counterpressure Sampling

Aseptic sampling without losing CO2. Descriptors: Counterpressure Sampling, shelf life of bulk beer.

It is necessary to use EBC Microbiology 2.2.1.2

  • 25/10/2018
  • 25
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  • Microbiological Techniques
  • |
  • 2011

2.2.1.6 - Sampling for Yeast Cell Counts

Collection of samples for the determination of the number of brewers' yeast cells (i.e. for an analysis of physical rather than microbiological characteristics). Descriptors: Sampling for Yeast Cell Counts, Copper sulphate

  • 25/10/2018
  • 23
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  • Microbiological Techniques
  • |
  • 2011

2.2.2.2 - Sampling of Thick Yeast from Closed Vessels

Aseptic sampling of thick yeast from a tank. Descriptors: sampling of thick pitching yeast from closed vessels, diaphragm, valve, sample cock.

It is necessary to use EBC Microbiology 2.2.1.2, 2.2.1.3 and 2.2.1.1

  • 25/10/2018
  • 27
  • |
  • Microbiological Techniques
  • |
  • 2011

2.2.3.4 - Neutralisation of Chlorinated Water

Preparation of sampling flasks for neutralisation of chlorine. Descriptors. chlorinated raw water, chlorine disinfectants, sodium thiosulphate